Closed storage of reagents and open storage of reagents with the prolonged storage time, both of the reagent blank absorbance are continuously increasing. This phenomenon is not mentioned in the kit instructions; within 12h of the two storage methods of reagent blank absorbance matching There was no significant difference in the t-test. Absorbance of both reagent blanks decreased continuously within 12h to 72h, but the decrease was slower. Closed storage of reagents is slower than the blank absorbance of the reagents stored in open space; at this stage, the reagent blank absorbance values ​​of the two storage methods are significantly different by the paired l test. For CO2 detection, CO2 detectors can be used for detection and analysis, providing convenience for relevant research. Plus Wechat controls more relevant information: Eva Mosaic Sticker,Colorful Mosaic Tiles,Mosaic Sticker Art,Creative Diy Handmade Mosaic NINGBO NEW SUCCESS ARTS AND CRAFTS CO.,LTD , https://www.craftandtoy.com
It can be seen that the absorbance value of the reagent blank has undergone a process of rising first and then decreasing, and the reagent specification has not been mentioned. Theoretically, the reaction of CO2 in the air with the reagent contained in the open bottle will only cause the reduction of N8 DH, so that the absorbance of the reagent blank will decrease continuously after reconstitution; the phenomenon that the absorbance of the reagent blank rises in the 121st flight after reagent reconstitution It is indeed puzzling.
In daily work, we generally refer to reagent instructions and require reagents to be re-dissolved at room temperature. After the reagents are poured on the machine, the reagents are not capped on the machine's special bottle, and only calibrated once a day before the sample is taken. The sample carbon dioxide concentration calculation formula [C sample 1 (blank absorbance-sample absorbance) allows [blank absorbance-standard absorbance] and standard concentration] to know that the default blank absorbance and (blank absorbance-standard absorbance) of the instrument remain unchanged after calibration, and actually Within 12 h after reconstitution of the reagent, the blank absorbance of the reagent has increased continuously with time. Therefore, after the instrument is calibrated for a period of time, the concentration of carbon dioxide in the sample is determined, and the measured result is necessarily low. The low degree increases with the increase in absorbance of the reagent blank.
Carbon dioxide detector for the reconstitution reagent reagent blank within 12h, the phenomenon of increasing absorbance, the author did the test of 1.2.2 above. This is based on the room temperature conditions. The serum was stored within 4 hours, and there was no significant difference in the results. Two-point end-point detection is better than the one-point end-point method. This is due to the difference in the calculation formula for the absorbance of the two detection methods. Obviously, the AI ​​change is greater than /l2. Similarly, according to the sample carbon dioxide concentration formula, one point end-point method can be used to reduce the result. Two-point end method. 