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Quantitative air spore catcher for spore collection and counting. Nine mature fruiting bodies were placed in kraft paper and 15cm diameter petri dishes (kraft paper and petri dishes should be sterilized first). Four spore caps were placed on the petri dish and placed in a constant temperature box of 5, 10, 15, 20, 25, 30, and 35°C for 18 hours to collect spores, and the spores were printed. The total area of ​​the individual spores was measured. After the spores were diluted to a certain concentration, the plate counted and the number of spores per square centimeter was compared.
The morphology of the spores collected from quantitative air spore traps was observed as the spores germinated and changed from the original near-circular shape to a narrow, elongated line to accommodate the need to migrate to the tubular mycelium. Under the fluorescence microscope, the nucleus of Flammulina velutipes spores is clearly visible. This is inconsistent with the description of many classic works about the nucleus of the spores of the velutin mushroom. The nucleus is relatively large. Two nucleus accounts for about two-thirds of the total spores. Nuclear and nuclear are very close together.
Quantitative air flow spore catcher for collection of spores of Enoki mushroom
The spores of enoki mushroom are the main materials used in experimental techniques such as cross breeding, spore isolation, and protoplast isolation and culture. The viability of spores directly affects the effect of these work, and the spore ejection and germination power is affected by temperature, medium and The influence of many factors such as its own physiological characteristics, etc. The application of quantitative air spore traps to the study of Flammulina velutipes spores is a method direction in recent years. It has been suggested that the spore nucleus of edible mushrooms such as shiitake mushroom and mushroom is more complicated.